Journal: iScience
Article Title: The Drosophila Him gene is essential for adult muscle function and muscle stem cell maintenance
doi: 10.1016/j.isci.2026.114670
Figure Lengend Snippet: HimGFP design and expression in leg disc myoblasts and adult MuSCs (A) Schematic of the HimGFP “minigene” comprising 3.8 kb upstream genomic sequence, mGFP fused to the Him coding sequence via an -SSSS- linker, followed by the Him 3′ UTR. (B) Larval T2 leg discs labeled with anti-Mef2 and anti-GFP show co-expression of HimGFP and Mef2 (examples arrowed) in the associated myoblasts ( n = 4). Scale bars, 100 μm. (C) Sagittal thoracic section showing an adult MuSC (arrows) on the periphery of a DLM fiber (boxed area shown at higher magnification). The MuSC expresses HimGFP and canonical marker Zfh1, and stains with nuclear marker Hoechst ( n = 5). Scale bars, 100 μm. (D) Close-up of a sagittal thoracic section showing adult MuSCs (arrowed) on the periphery of a DLM fiber that express both HimGFP and Mef2 ( n = 3). Scale bars, 20 μm.
Article Snippet: Rabbit anti-Mef2 (1:1,000) , DSHB , Mef2.
Techniques: Expressing, Sequencing, Labeling, Marker